The aim is for students to acquire good knowledge of fundamental structures and biochemical processes that will help them understand the biochemical structure versus function relationship, the physiology and pathology associated with certain dysfunctions and sustain them to better understand the contents of other disciplines such as microbiology, hematology, immunology, physiology, pharmacology and toxicology, as well as the acquisition of the necessasry lab methodology to the evaluation and study of biomacromolecules.
Theoretical Syllabus
Macromolecules (proteins and carbohydrates), and its monomers (sugars and amino acids): structures, physicochemical characteristics and functions in living been. Metabolic aspects including enzyme catalysis and the free energy associated to the biochemical reactions; biosynthesis and degradation of glycogen, glycolysis and gluconeogenesis, via oxidative of phosphogluconate, the Krebs cycle, the electron transport chain and oxidative phosphorylation.
Laboratory Syllabus
Study aminoacids and potentiometric titration; methods of protein quantification. Electrophoresis. Kinetic study of enzymes (determination Km, Vmax); Evaluation of the effect of pH, temperature and inhibitors. Determination of Ki.
The component Theorical (T), expository, addresses the theoretical concepts with concrete examples of current examples of experience and practical; the practical component (PL), involves performing laboratory techniques which are related with T. Moodle platform offer up educational and support content.
Berg, J.M., Tymoczko, J. L., Gatto, G. J. & Stryer, L. (2015). Biochemistry (8th ed). New York, NY. Freeman/Macmillian.
Nelson, D. L. (2012). Principles of Biochemistry (6 th ed). New York, NY. Freeman.
Quintas, A., Halpern, M. J. & Freire, A. P. (2010) .Bioquímica - Organização Molecular da Vida (1 th ed). Lidel
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