To provide a solid training in Molecular Genet ics in order to understand the diverse applications of this scientific area
in veterinary medicine.
Theoretical cl asses:
1. Nucleic acids
2. Techniques to manipulate nucleic acids
3. DNA replication and molecular basis of r ecombination
4. Gene expression: transcription
5. Gene expression: translation
6. Gene expression regulation
7. Mutations and DNA repare m echanisms
8. Molecular markers
9. Restriction mapping
10. Sequencing
11. Microbial gen etics
12. Recombinant DNA technology
13. Genetically modified animals
14. Animal cloning
15. Aplications of M olecular Genetics in modern Medicine
Practical lessons:
1. Practical work: D NA Extraction, evaluation of quality and quantity parameters of the extracted DNA.
2. Practical work: Molecular techniques using polymerase chain reaction (PCR);
3. Practical work: Molecular techniques using restriction enzymes digestion;
4. Practical work: Molecular techniques using real-time PCR;
5. Resolution of exercises: Restriction mapping;
6. Resolution of exercises: Sequencing.
The theoretical lectures are presented with power po int presentations. Classes PDFs are previously published in the SIDE platform. In the classes, in addition to the exhibition by the professor, the discussion of the various syllabuses is promoted and the students are encouraged to submit their questions. Classroom: Auditório do Edifício das Pedrinhas. The practical classes consist mainly in the application of theoretical concepts by performing experimental protocols. The works are held in groups of 3 to 4 students. Theoretical-practical classes with resolution of Molecular Genetics exercises will also be taught. Classroom: Lab room 2.13 Edifício Geociências, where all the laboratory equipment needed to carry out practical protocols are available. The evaluation of this UC is made exclusively by Exa mination Mode 3).
Brown T.A. (2010). Gene Cloning and DNA Analysis : An Introduction (6th edition).
Reece, R. (2004). Analysis of genes and genomes.
Watson J. et al. (2013). Molecular Biology of the Ge ne (7th edition) .
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