In this curricular unit (CU) students must purc hase a set of skills that are large by providing them knowledge that can be used in other UCs of plan of studies of this masters. In addition to the knowledge of the physicochemical properties and functions of biomolecules acquired in curricular unit of Structural Biochemistry, taught in 1st semester of this master's degree, in the CU of Biochemistry of Metabolism will be explored inter-relationship of these compounds in the set of reactions that occur in the cell (metabolism). Additionally, this study of cellular metabolism will be directly related to information associated with more advanced areas of pathology, toxicology and pharmacology. In practice should be able to use the more basic techniques for analysis of metabolic processes: spectrophotometry,
electrophoresis, titrimetry, chromatography....
1. Bioenergeti cs and Introduction to metabolism.
2. Metabolism of carbohydrate: glycolysis and Ne oglucogenese; Krebs cycle; Mitochondrial respiratory complex, electronic transport and oxidative phosphorylation; Pentose-phosphate pathway; Uronic Acid pathway; Biosynthesis and degradation of glycogen.
3. Metabolism of lipids: catabo lism of fatty acids; anabolism of fatty acid; biosynthesis of triglycerides, phosphoglycerides and sphingolipids; biosynthesis of cholesterol and control mechanisms: biosynthesis of derivatives of cholesterol, bile acids and steroid hormones; transport of lipids: metabolism of lipoproteins.
4. Metabolism of nitrogen compounds: catabolism of amino acids; biosynthesis of amino acids; biosynthes is and catabolism of purines - control mechanisms; biosynthesis and catabolism of pyrimidines. Biosynthesis of ribo- and desoxirribonucleotidos.
5. Integration of metaboli sm.
The theoretical classes are based on oral exposure s upported by multimedia presentations. Will be given to students the opportunity for discussion all topics relevant to the content being taught in the classroom. All the discussion will be moderate by the professor.
Practical classes will be taught in the lab, where students will have to run experimental protocols in structural biochemistry, and these, whenever possible, will be associated with the matters to be taught in lectures. Practical work
will have a brief theoretical introduction based on oral presentation by the teacher. In practical component of metabolic biochemistry, the practical implementation of t he protocols, as well as the discussion of the results, is conducted by students but supported by the teacher. To the students will also be available in advance all media files presented in class as well as the protocols used in practical classes. Students are evaluated in continuous, additional and exam evaluation.
Engelking, L. R. (2011). Textbook of Veterinary Phys iological Chemistry. 2nd edition.
Harvey, R.A. & Ferrier, D. R. (2011). Biochemistry. 5th edition.
Lieberman, M., & Marks, A.D. (2009). Basic Medical Biochemis try - a clinical approach. 3rd edition.
Nelson, D.L., & Cox, M.M. (2008). Lehninger Principles of Biochemistry. 5th edition.
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