It is intended that students develop and complement the basic knowledge acquired in practical classes of the discipline of microbiology. To develop knowledge in the use of appropriate techniques for isolating microorganisms from various samples, for their characterization and for techniques allowing identification. It is also intended to provide students with skills that enable understanding of some techniques used in quality control and evaluation of antibiotic sensitivity, as well as the appropriate safety procedures in a microbiology laboratory. At the end of the classes students are expected to know how to maintain appropriate attitudes in the microbiology laboratory, applying the knowledge acquired in laboratory safety, develop an aptitude for teamwork, develop habits for maintaining proper records of all procedures and to interpret the experimental data resulting from the different techniques.
Introduction to the discipline: standards of conduct and safety rules; laboratory notebooks as a way of recording procedures.
Observation of bacteria in mo: staining techniques (Gram, negative, endospores); observation of preparations with other staining techniques and phase contrast mo.
Isolation and growth of microorganisms: culture media; isolation techniques; influence of physical factors; cultural characterization; Maintenance of cultures.
Biochemical characterization and identification of bacteria: metabolism of carbon compounds and nitrogen compounds; respiratory enzymes; extracellular enzymes; growth factors; Kits used in identification.
Identification with independent growth methods: molecular methods; Use of antibodies.
Isolation and characterization of sporulated bacteria from soil samples.
Bacteriological examination of water and milk.
Evaluation of the sensitivity of bacteria to antibiotics: the agar diffusion technique; Determination of Minimum Inhibitory Concentration.
The TP component integrates the presentation of the theoretical foundations, techniques, and experimental design. A methodology for interactive exhibition will be followed and stimulating discussion of the topics.
In P component, students undertake practical work, using the techniques discussed and following the available protocols. The work is performed in groups and with accompaniment. Students develop work in all components of the techniques used, through the organization of work, the preparation of all materials, the execution of techniques, observation, analysis and discussion of results.
Each student keeps a lab notebook to record all procedures and to be use in all classes.
Willey J.M., Sherwood L.M. & Woolverton C.J., 2014, Prescott's Microbiology, 9ª ed., Wm. C. Brown Publishers.
Wanda F. Canas Ferreira, João Carlos F. de Sousa e Nelson Lima ed. 2010. Microbiologia, Lidel, edições técnicas.
Alcântara, F., Cunha M. A. e Almeida M. A. 2001. Microbiologia : práticas laboratoriais. Aveiro : Universidade.
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