Operational Methods of Sea and Laboratory I

Objectives

1. To know and apply some of the methods used in biological oceanography (sensu latu), including laboratory techniques and data analysis.

2. To develop the ability to collect data, analyse them and prepare a scientific document

3. To learn laboratory techniques, applying them to study and draw up reports or documents for dissemination of the results obtained.

Program

1. Types of data used in fisheries:

Random and stratified sampling

Practical application to fish landings

Laboratorial biological sampling

Statistical analysis of biological laboratorial data

2. Histological methods:

Collection, fixation, inclusion, cuts

Staining techiques

Observation of biological structures

3. Laboratory practice in quality control of fish (fresh and canned):

Use of different laboratory equipments

Analysis of salt and water contents

Determination of heavy metals (mercury) and histamine

4. Extraction of bioactive compounds (biopolymers) in sea anemones:

Collection and preparation of samples

Determination of proteins, lipids and carbohydrates

Search of bioactive molecules

5. Cetacean photo identification methods:

Photographic equipment and techniques

Image selection and analyses

Identification of individuals and resightings

Models to estimate population size

Teaching Methodologies

The content will be provided to students on the basis of theoretical and practical lessons, including classes in person lab or in the classroom for analysis and processing of data. Students will have the opportunity to follow and participate in laboratory work where researchers develop experimental research actions. Theoretical and practical lessons may be invited researchers, experts in specific subjects to collaborate on specialized themes transmission.

In the analysis of data will be used in data analysis software (e.g. Excel, Statistica) which will allow you to perform statistical tests and elaborate graphics quickly.

Bibliography

Shick JM (1991) A functional biology of sea anemones. Chapman and Hall, London

Lodish, H. et al. (2004). Molecular Cell Biology (5th ed.). New York, New York: WH Freeman and Company

Acober, L.F.; Rand, A.G.J (1982). Biochemical evaluation of seafood.

Martin,R.E. (1982). Chemistry and biochemistry of marine food products. Connecticut: Westport,. Cap. 16 p.347-365.

OMS (1990). International Programme on Chemical Safety.Environmental Health Criteria 101. Methylmercury. Geneva.

Russel, F. E.; Maretic, Z. (1986). Scombroid Poisoning: mini review case histories. Toxicon, 24 (10): 967-973.

Soares, V.F.M. et al. (1998) Teores de histamina e qualidade físico-química e sensorial de filé de peixe congelado. Ciência e Tecnologia de Alimentos, Campinas, (4): 462-467.