1. Understand the structure and organization of chromatin and genome
2. Know the mechanisms of regulation and control of the genome, gene expression
3. Understand the mechanisms of gene regulation with the associated structural and numerical changes.
Understand the role of fusion proteins
4. Understand the histone code and epigenetic modifications
5. Understand the failure of cycle control systems and signal transduction control
6. Understand the function of tumor suppressor genes
7. Understand the changes in the intracellular signaling pathways, growth factors and their receptors
8. Understand the genome structure and its relationship to expression proteomics and its physiological consequences
9. Understand the various techniques of genetic analysis
1. Introduction to Molecular Biology - The purpose of Genetics and Molecular Biology (GBM)
2. Organization and structure of the human genome
3. Study of the mechanisms of epigenetic regulation
4. Genetic Bases of Cancer: the regulation of the gene expression. Types of Mutations
Changes that attach or remove functions
Oncogenes and Proto-oncogenes
Inherited mutations of tumor suppressor genes
Changes in signaling pathways of the cell cycle control
Changes in growth promoting proteins
5. Tumor Cells and Cancer Appearance
Properties and changes in gene function. Metastases
Cancer stem cells
Hypoxia and Angiogenesis
6. Main techniques in molecular biology
7. Pratical sessions in molecular biology.
Extraction methods for genomic DNA and plasmids
DNA analysis, restriction analysis of plasmids.
Interactive methodology, combined with the analysis and discussion of integrative themes of the theoretical and practical concepts
On each of the program's topics will be provided a presentation in PPT and the bibliography necessary for the interpretation of topic
Students are encouraged to participate in class, giving the questions and discussing with each other (and the teacher) the topics
In the laboratory classes, the students explore and execute protocols for the extraction and analysis of DNA.
Molecular Cell Biology. Lodish, Berk, Kaiser, Krieger, Scott, Bretscher, Ploegh e Matsudaira. 6ª edição (2004). W. H. Freeman and Company. Capítulos 4, 23.
Molecular Biology of the Cell. Alberts, Johnson, Lewis, Raff, Roberts e Walter. 4ª edição (2002). Garland Science. Capítulos 5, 6, 23.
Biologie Moléculaire. Maftah e Julien. 2ª edição (2003)Dunod, Paris
Biologia Molecular e Celular. Stansfield, Colomé e Cano. (2000). Biologia Molecular e Celular, McGrawhill, Lisboa
Gene Cloning, an introduction. T.A. Brown. 6ª edição (2006). UMIST, Manchester
Essential Molecular Biology, A practical approach, (Volume I e II). T.A. Brown (2006 e 2008). IRL Press, OUP, Oxford.
Hiperligações:
• www.biocarta.com
• www.pathwaycentral.qiagen.com
• http://www.nature.com/
0201514
4